Hi Cell Profiler community.
I’ve read the previous forum posts about H&E pipelines but I can’t seem to get mine to work.
What I want to do is get an average cell size by counting the amount of eosinophil stain and dividing by the number of nuclei. Even better would be if I could divide total tissue size (eosinophil and nuclei) by number of nuceli.
attached is my pipeline and the image i’m trying to interpret. I know I’m quite a way off but was hoping someone might be able to point me in the right direction!
*please see newer pipeline and higher mag image in comment below