I’m interested in using CellProfiler to measure the transport rates of particles moving along axons. These particles frequently move under/over one another, which has so far confounded all automated tracking techniques. I’ve been generating kymographs of the these axons and measuring the particle movement rates, stall durations, and reversals manually, but this is slow and tedious.
I downloaded the example pipeline for sorting what looks like mitotic cells, but it didn’t seem to work well for my movies. What’s a good way to get started on this, remembering that the particles I"m interested in principally move along a single axis? I can submit example movies/images if needed.
Thanks for your help!