Having the Scale Bar on a separate layer than the background (original image)?

I would like to edit the saved TIFF image in Photoshop or another raster editor, so is there a way the Scale Bar and the associated text can be saved on a separate layer?

I tried the Overlay option, but then I can not see the scale bar in raster editor.

Good day,

ImageJ/Fiji do not support layers.

What I don’t understand is, why you want to do further image processing outside ImageJ. Nearly everything you can do with Photoshop can be doen with ImageJ/Fiji .