Hi my name is Damon Cargol and I’m an employee of the University of Washington’s department of biochemistry. Richard Frock and I came up with a pipe for quantitating the number of nuclei per area in H & E stained muscle cells (see the description below and attached pictures). We thought it might be useful to others, like your example page, and wondered if there was a specific place to upload files for the community of cellprofiler users. I’ll attach it here too.
“We were interested in attempting to quantify our Hematoxylin and Eosin stained skeletal muscle sections in a fast and unbiased way. In particular, we focused on quantitating total nuclei in an image of a given muscle section by dividing out the undesired features and retaining only the nuclei. We also wanted to make a comparison against similar tissue of different genetic backgrounds and given that each image represents an amorphous area of tissue with possible gaps or ends displayed, we were able to normalize the nuclei count based upon the pixel area that was analyzed within each image.” –R. Frock
Sample Images -------------------