Thank you for all of your work in developing this amazing software and for providing help to us through this forum.
I’m currently having an issue with using a pipeline to count the number of cardiac cells in a histological H&E image (and through doing that, find the cell density in that image). I’ve been able to develop/modify a pipeline accomplishing what I desired. Basically, the pipeline counts the number of nuclei in the image, and I use that to represent the number of cells. However, I’ve tried running the pipeline on 20+ different images; and while it works well on some images, on others, it is unable to count any nuclei. I’m not sure what is wrong with the pipeline; I’ve tried adjusting threshold values, diameter size, and everything, but its effectiveness is very variable.
I’ve attached the project with the pipeline and three images. The image, labeled with Rat 81, works well with the pipeline. The two images, labeled with Rat 90, are examples of the problem I encounter (from a nuclei count that makes little sense or no nuclei count at all).
Is the issue with the quality of my images? Or is there something that I need to change with the pipeline to make it work better?
Once again, thank you for your time and consideration.
CellDensityHL(working).cpproj (157 KB)