Am I right in saying that DAB stains for intracellular (but not nuclear) proteins and Haeamtoxylin only stains for nuclear proteins only. I am analysing cytosolic proteins (PLOD2 and NICD) and RhoE (which can shuttle between the nucleus and cytosol). I’m a novice to QuPath and I’m thinking that the best way to analyse the expression of these proteins would be to create a threshold for DAB and Haematoxylin and then run positive cell detection?
Am I right in saying that ‘positive cell detection’ just includes cells that meet the DAB/Haematoxylin threshold?
Any help would be much appreciated!