I need to write a macro that can look for circular grains in a field of view on an agar plate. The dimpling on the plate is making it complicated to discern individual grains when they are clumped together. And the dimple in the agar is making things tricky. Can anyone help?
Typically I would try a macro like this:
run(“Subtract Background…”, “rolling=50 light”);
run(“Analyze Particles…”, “size=1150-Infinity circularity=0.6-1.00 show=Overlay exclude clear summarize”);