I am having difficulty figuring out how to use imagej in order to analyzed a grey scale image for particle thickness distribution. We are analyzing thin, few layered and monolayer MoS2 particles. Thickness has a direct correlation to the number of layer within the MoS2 particle.
Is there a way to analyze a microscope image of these exfoliated particles and create a distribution of thickness based on the greyscale values of the image?
Any help is greatly appreciated!!