I am having trouble with Fiji on Windows 7. I have been setting the scale to 10 pixels per 4 micrometers (2.5 pixels/micrometer) and checking the box that says “Global”. If I close the image and use DiameterJ to segment it, the scale is reset to the default (96 pixels/inch). If I reopen the image, I get a message that says “The calibration of this image conflicts with the current global calibration.” I have searched online, and cannot seem to find any troubleshooting tips for this issue. Has anyone else experienced anything similar to this?