I am the initial stage user of ImageJ. Here I am having a problem, In case anyone could help out that is great.
I have an Rois in an Z-stack got from some masks. Now I want to pick the raw intensities of these ROIs from the raw image i.e. I need an image of same size (don’t want to crop it) with the raw intensity of the ROIs only. Is there a plugin or option to do this in ImageJ? Or should we write our own code to do so?
Basically other that ROIs pixels, rest have to become 0/1.
Here I am sharing the Roi file & my raw image file with the Rois in it.
RoiSet.zip (42.3 KB)
Below is the cropped image