I’ve found a code to make multiple line ROI segments from a single drawn line. With all lines (~500) in the ROI Manager however, how do I make the intensity profile across all of these lines, the same way I would individually select each ROI & hit Ctrl+K (Analyze -> Plot Profile), and gather the data of each coordinate on that graph?
I should clarify that mean gray scale values and min/max values are not enough for the analysis that my team is doing. We really do need intensity values of every pixel across the ROI.
Thank you for your time!