FracLac plugin - how do I use it analyze intensity around a cell?

I am new in image analysis.
I am trying to analyze localization or non localization of a protein around a cell. Thus, I am trying to measure the intensity around the cell between my experimental groups. I read that I can use box counting method on the FracLac plugin but I do not know the steps to take.
I do not know how to make my images like in this link

Dear @koko,

as far as I know, the author (Audrey Karperien) of FracLac is not active in this forum. You should try to contact him to get further assistance.


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