I am using the Fourier ring correlation plugin from the BIOP site. I have two confocal images from two APDs separated by a 50/50 beam splitter. The signals are from the scattering of gold beads. After running the plugin, I obtained a result like this. There is a huge amount of high-frequency components and a “resolution” cannot be determined. Does anyone have an idea why this happens? Sorry I cannot upload the original tiff images because of the restriction of the forum.