I’m trying to analyse TIFF files by attempting to create a pipeline using CellProfiler. For easy viewing purposes, I have uploaded JPEG examples below:
(Where Image 1: Astrocytes visible due to GFAP antibody,
Image 2: Neuronal cells visible due to NeuN-biotin Antibody,
Image 3: DNA nuclei visible due to DAPI,
Image 4: [Composite image])
However, I have been unsuccessful in creating an appropriate pipeline to identify each separate entity (astrocyte/neuronal cell/DNA nuclei) and am then further wanting to undertake quantitative analysis, i.e. how many astrocytes, neuronal cells, DNA nuclei present, so I can export to Excel and carry out statistical analysis (bin analysis - most likely).
Is anyone able to help please? I’ve attempted to watch so many tutorials and use examples but nothing seems to work.