I tried many times on my own pipeline and the examples from CellProfiler as well, but the problem remained. After ExporttoSpreedsheet, all the data is always stuffed into the A column. Could you help me?
Another question is that if I want to directly obtain the diameter instead of the area of circular speckles, how should I set the parameters in CellProfiler?
The last quesion is that can CellProfiler process .DAT file (text image) directly? Currently I am doing it in this way:
Export the images from the microscope software as .DAT;
Import as “text image” in Image J and then resize;
Batch process in DeconvolutionLab (a plugin)
Batch convert to 8 bit .TIF;
Analyze in CellProfiler…
I wonder if there is some better way to do it?
Many thanks for your time and help in advance!