The following is from a colleague here at MBL:
I’m a student in the MBL Neurobiology course and we are setting out to use DeepLabCut to analyze fly larvae behavior. We are planning to run DLC with 18 points placed around the larva to characterize behavior in response to stimulus, such as C-bending, S-bending, elongating, and rolling. I wanted to see if you have advice on how to perform this analysis? We were thinking about calculating all pair-wise distances between the points and to cluster them with tSNE + NNMF, then attempt to pull out individual frames to see and describe the behavior. Our main goal is to construct a raster plot of larva behavior over time. Any suggestions or advice would be really helpful!
Chris & Doug