Fluorescent bead tracking in DIC image

Hi,

I am trying to find the best way/plugins to track multiple fluorescent beads moving with cell in a media to get its velocity and directionality from DIC image.
I used to track one bead at a time in an avi file and looking for a plugin that allows me to do multiple beads tracking at a time of an avi file.

The image is like as below:
bead2

The line is the trace of the bead distance moved manually tracked with the multi-point tool in imageJ.
This is very time-consuming and inefficient way so I am hoping to get advices or recommendations for the optimized way for this type of data collection.
Thank you!

Perhaps TrackMate? Which comes with an extensive manual :slight_smile:

Thank you. I will try TrackMate!