Fluorescence Intensity

Hi, so I’m trying to quantify the amount of fluorescence in my images but the statistics I am getting do not match what I think they should be based on the images. Many images seem too high/low based on both the total intensity and mean intensity statistics. For example: well B2 (attached) places near the top of the statistics but there is hardly any fluorescence visible. It places higher than well C4 which seems like it should be higher intensity. I also cropped the images in the pipeline to avoid the halo visible mostly on the left edge.
Green.cpproj (93.3 KB)

Nevermind. Confused Integrated Intensity with Mean Intensity

No problem, glad you straightened it out.