I have 2 types of images. One image show fluorescence intensity of the membrane and other in mostly cytoplasm. I would like to know if I can analyze the fluorescence intensity and segmented the membrane in the two images. I am new to cell profiller and I am trying to learn to analyze my images.
cytoplasm.tif (1.2 MB) membrane.tif (978.2 KB)
I tried the analysis with the QUIMP plugin on FIJI but the plugin changes my 32-bit image to 8 bit so it creates results with a different scale.
I would like to set up a value for the membrane so then I can analyze all my images with the same membrane size.
Please, see attached the images.
Thanks in advance for any help