I am trying to take picture of immunofluorescence reaction with Micro-Manager, but I am having a lot of problems with it. We have a Retiga 2000R from QImaging. It is a mono camera, but we have a cube for fluorescence attached to our Leica microscope to see fluorescent staining. I already installed the QCam driver on the pc and MM recognize it and let me see my slides. However, I can’t find a way to set channels or pseudocolors in order to see the fluorescent staining correctely. When I tried using the Multi-D acquisition, I obtained very “dirty” and blurred images. Is there a way to fix it and finally take good pictures?
Thanks a lot in advance for the help!