I have some problem with opening flex files containing 2 images -RFP and DAPI. My folder contain 36 images, ex. 003002000.flex, 003003000.flex etc.
After LoadImages module I have GroupMovieFrames module to split two channels to separate images. The analysis is working fine, but the name of images
is not correct. For some reason the Loadimages module give the name 00300200.flex_1 instead of 003002000.flex and in the next cycle 003002000.flex_2
instead of 003003000.flex, then 00300300.flex_1 instead of 003004000.flex. Surprisingly the names in GroupMovieFrames do not correspond to those in
LoadImages. For example in cycle 2 in LoadImages it says 003002000.flex_2 (instead of 003003000.flex), but in GroupMovieFrames is 003003000.flex_1 and
003003000.flex_2. But the names in the output files correspond to names in LoadImages module.
Could you help? Thanks a lot.
I am running CellProfiler 1.0.7522 on MacOS 10.6.2. I tried also to upload some flex files but “The extension flex is not allowed”. SO I attached just my pipeline.
SKN2RFP_Cellmask_100330PIPE.mat (1.37 KB)