I would like to announce the release of the ParticleSizer script:
The ParticleSizer script was developed to automatically measure the distributions of characteristic size and shape properties of a nanomaterial. In the scope of implementing the European Commission definition of a nanomaterial, the minimal external dimension of the primary particles of a particulate material is assessed as the minimal feret diameter from electron microscopy images. Other size and shape parameters are measured simultaneously.
During the development, we used the large image database of the NanoDefine project ( www.nanodefine.eu ). The ParticleSizer combines several existing ImageJ plugins (e.g. background-subtraction, non-local means, shape-filter, ellipse fitting) but also introduce some new ideas (e.g. to remove artifacts from segmentation, detect single particles). The focus was, to provide a robust tool to rapidly estimate the size distribution from TEM images without much user knowledge about image processing. However, when it comes to more complicated images, it is flexible enough to adapt to those situations.
You can find more information here:
After publication, much more information about the pipeline will be added in the wiki.
I’m would be happy to get some feedback.