I am trying to find the cells in attached image.
- First of all: any ideas are welcome!
- I tired the Enhance Dark Holes algorithm and it works quite well for cells where the cytoplasm completely encloses the nucleus. However cells where the cytoplasmic ring is not complete are not found. I think this could be because in your algorithm you do subsequent erosion and reconstruction; my feeling is that the algorithm might become robust to incomplete rings if you replace the reconstruction by a simple dilation, such that you basically perform subsequent steps of classical opening operations. Could you try this? Or, even better, give the user the choice whether they want to use erosion>reconstruction or erosion>dilation?
- More general comment: Find Dark Holes is a granulometry filter (with reconstruction) on the inverted image. I think it would be great to add a simple Granulometry filter (with min and max particle radius) to Image Morph!!