I’m trying to use QuPath to analyse imaging mass cytometry data. I have tried to import images in different formats (16bit and 32bit multi and single tiff from MCDViewer, .mcd and txt) but I can’t manage to import one single file with all the channels included as required for the multiplexed analysis. I have seen that the sample data used in the QuPath tutorial is either tif or qptiff (not sure about it).
I’d be very grateful if you could answer these two questions:
Which formats can I use in QuPath for imc multiplexed analysis?
How can I transform my Hyperion files which are .mcd in any acceptable format for QuPath?