I am using QuPath 0.2.0.m9.
I am using DeltaVision Systems to image the whole slide with 20xOil lens. Tissues were stained with four markers (including DAPI). After acquiring images in all the four channels, I deconvolve, stitch and max intensity project.
I have noticed one thing regarding importing big images in QuPath, if the file (max intensity projected) size is bigger than 2GB, it gives an error. Also, in the images tab, image name appears as “Null image”.
Here is the link for one of the sample image with a size of 3.29GB. I able to open this image with DeltaVision software and Fiji. (https://drive.google.com/file/d/1x2jCD_fs7mXJt4dz8NVfzYtMJkUcvCbk/view?usp=sharing)
Snapshot from Fiji