I am new to Fiji and scientific image editing (helping a scientist post scans of microscopic slide online) and would appreciate if someone can give me step by step instructions regarding following situation:
- I have .nd2 files that need to be converted in such a way to preserve all characteristics of a stacked image and be very similar ( the same) as what students would see using a microscope. I used Bio-formats plugin to import the image into Fiji, but am not sure what the next step should be. If I just export it as a tiff (using Bio-formats) it flattens the image. How do I ‘unzip’ .nd2 image into stacks and what do i do to export it in a correct way?
Thank you very much for the help,