FIJI 3D-OC Volume measurement- Heart

Hi All,

I need to measure the volume of a heart image (170 x 170 micron, 50 stacks, 1 um separated). I used the fluorescence and size threshold so that the 3D-OC counts the whole heart as one object and it also give me the volume of that one object (which is the heart).

I just want to be sure that my method is correct.

Any insights will be hugely appreciated.