I have created several rois using thresholding, “Set Measurements…”, and “Analyze Particles”. I’ve combined them using “OR (Combine)” in the Roi manager and would like to measure the Feret diameter of the resulting combined roi. This works; however, the coordinates given for the Feret diameter (FeretX, FeretY and FeretAngle) are always 0. This is not the case if I measure rois that are not combined rois.
Is there any way to get around this problem? I particularly need the FeretAngle to make my analysis useful.