I have a feeling this is a common problem. When I’m using the identify primary objects tool, because the image is black-and-white and there are sometimes shaded regions in the cell, the computer thinks that it is TWO cells and thus, I have a false positive number. If I increase the minimum allowed distance for local maxima, then this will cause some other cells to have a false negative (because they’d be too clumped together.
What should I do? Attached is my pipeline and a picture.
Still_Testing_DAPI_1.cp (10.2 KB)