Hello! I’ve attached the first 4 files of an image set of 12 (b/c Im a new user I can only post 5 at once) I’m attempting to tile from the EVOS m7000. The software is silly bc it collects the images starting at the middle of the well, and in a “serpentine clockwise” position. When I try to tile using CellProfiler, I can only select starting from the corners. I keep getting mis-tiled images or black files exported. I’ve tried telling it the number of rows or columns, to help it along but nothing is working. There aren’t many options in this module, so I’m wondering if anyone has suggestions or if i"m better off with imagej. I just don’t konw how to batch process these files on image J, considering there are multiple timepoints, and channels in the same folder. Thanks in advance for any help you can offer.
21-01-29_Plate_R_p00_0_A01f00d4.TIF (5.4 MB) 21-01-29_Plate_R_p00_0_A01f01d4.TIF (4.9 MB) 21-01-29_Plate_R_p00_0_A01f02d4.TIF (5.2 MB) 21-01-29_Plate_R_p00_0_A01f03d4.TIF (5.0 MB) tiling.cpproj (831.8 KB)