Hello, is it possible to extract 4 channels from an image using ImageJ, i.e., blue, green, red, and far-red, or will the red and far-red channels always be merged? Thanks.
Too - I’d recommend opening your images in Bio-Formats (if you don’t already). That way you are sure your metadata is read correctly and your image properly opened, etc.
If you have more specific questions - don’t hesitate to ask. We are all here to help!
Thank you for your kind help.
Hello again, I’ve looked at the user guide and tried opening my images through the Bio-Formats plugin but can’t seem to locate any option that recognizes 4 channels (blue, green, red, far-red) instead of assigning only 3 (blue, green, red). Any more guidance would be greatly appreciated. Thanks.
I don’t think you have yet mentioned what kind of images they are (czi, tiff, mrxs, 4 vs 3 channel within the image itself, etc), and whether the image type is Bio-Formats compatible. If you are using a processed image, you may have lost the fourth channel at some point because the image was saved in an RGB format. More information is needed.
Providing a sample file is also suggested in the initial forum post text.