My name is Wim Trypsteen and I am a graduate student at Ghent University.
I have a specific imageJ image processing question:
I need to extract fluorescence intensities from a tiff file that has a picture of a slide with about 3000 circular spots.
How can I do this in ImageJ if at all possible?
This would require making a stencil/layout, overlap this with the tiff image and extract the fluorescent values of those 3000 circular spots.