I’ve been using bigwarp to align TEM images with confocal images, but I’m having trouble exporting the fused image after alignment with the resolution of the TEM image. I’m using the TEM as a moving image and the confocal as target image, both 8-bit TIFFs.
When trying to export the moving image using File->Export as ImagePlus it gives me the options to export the resolution and field of view with either the moving or target image, but after trying out all different options and combinations I can’t seem to get the resolution from the TEM moving image to the exported image.
I’ve then tried this script from the ImageJ homepage to get the moving and target image aligned using my landmarks (bigwarp/Apply_Bigwarp_Xfm.groovy at 90cc95f5fe5cf01408a42b292b195d31ff866acf · saalfeldlab/bigwarp · GitHub) but I get an error message “Undefined variable in line 8”. I’ve also tried just capturing the transformed image from ImageJ utilities menu, but it doesn’t work either (won’t register bigwarp windows while using the plugin)
I’m sure the solution to my problem is simple and there is something I’m missing here…