Hi @Stephane and @MarionLouveaux,
Thank you both very much for your help! I was able to use Spot Tracking without Spot Detector using both of your methods.
There is one other issue I’m running into. While I am able look at the tracks from my segmented image using Track Manager, I also want to look at the area of these spots over time. However, I keep getting an error when I try to use the ROI Statistics Track Processor in Track Manager.
I have been using this protocol that I saved in order to add my segmented areas to ROI and to Swimming Pool:
However, whenever I then use Spot Tracking (both the Simple and Advanced Interfaces) and Track Manager and then try to use the ROI Statistics Track Processor, I get an error like this (sometimes the message varies in the min/max values it lists):
I was wondering if you knew how I could resolve this issue? I’m not sure what “bounding dimension 2” is referring to and how I could change those values.
Here is a sample of an image I segmented in Ilastik that I am trying to track and measure the flares of:
0017__mC2xrGBD10_Simple Segmentation_LowerLeftQuad.tif (11.5 MB)