Hello, I am using cellprofiler to measure the total intensity of fluorescence in a set of microscope images. My problem is that most images have more than one cell and when the numbers are exported I have a list that tells me the number of cells in each image and the total fluorescent intensity for all the cells, but these numbers are combined. I would like to use standard deviation or other statistics on my numbers, but I need the individual fluorescent values to do this. Any suggestions?
I attached some samples images (positive and negative) and the pipeline I have been using for this analysis. Thanks!
FinalDigitoninQuantify.cp (7.69 KB)