Sorry if this is a silly question but I am fairly new to Cellprofiler and I am also not well versed in programming. So attached is a pipeline for counting RFP and GFP foci and their colocalization. One issue I had is that the results get put out in an Editor text file and not in an Excel file. Does anyone know how I can fix that ? As I said I’m fairly new to the topic and the pipeline still needs a lot of work. Apparently it has something to do with the column delimiter being “Tab” instead of “comma” but since I want the data seperated in tabs I would prefer to keep it that way. If anyone has tips for general improvements it is also really appreciated.
I have also attached 2 images that I use for testing the basic functions of the pipeline so you can test it for yourself.
Version: Cellprofiler 3.1.9
Test images for import into pipeline (Normal yeast cells, no foci):
FUS manual verification 0h fixed 060220_A01_s1_w2.TIF (2.8 MB) FUS manual verification 0h fixed 060220_A01_s1_w1.TIF (2.8 MB)
Pipeline Coloc_RFP and GFP foci.cppipe (38.9 KB)