I was wondering what SPIM users are using to register their multi-view datasets captured at different angles. If using the Multiview Reconstruction plugin in FIJI how easy do you find this, do you have any general advice about how to make it more likely registration is successful?
We’re finding that our sample changes position considerably when it is rotated so quite big changes in xyz via the sample stage are required and can even mean there is no xyz coordinate overlap to bring the sample back to the same focus once rotated, this means we have to manually try to overlay the datasets in the Multiview reconstruction plugin before even starting to detect interest points. How much of this can be alleviated through accurate sample mounting e.g. the sample is close to the centre of rotation of the rotation motor by being in the middle of whatever vessel is used FEP tubing etc.