I have a mixed population with GFP labelled and unlabelled cells. Now I want to exclude the GFP positive cells and go on with the analysis only with the unlabelled cells only. I Identified first the nuclei then using secondary identification the GFP labelled cells. Now I want to cut the GFP positive cells away.
With CP1 I used Module Exclude and now in CP2 it is called MaskObject.
My Problem is: I want to keep the nuclei OUTSIDE of the masked region, not inside of this. In other words I want to use the nuclei that are not in the GFP positive cells. Is there a tool to invert the region or could you build a additional feature to choose exactly to ones outside the mask?