Does anyone know of a way to exclude certain features in images based on their thickness please? In the image below you can see large tracts (often with small circles in indicating where the cell bodies are) with thin vertical fibers in the spaces in between. I would like to find a way to exclude the thin, vertical fibers (e.g. based on thickness) as they are currently confounding my analysis.
Ideally I would have another biological marker that did not express in the thin fibers that I could use as a mask, but I’m not aware of one right now.
I have tried using the Local Thickness plugin to try to generate a map of thick fiber locations to then use that as a mask, which worked to some extent, but ended up excluding some other bits too.
Any help much appreciated