I’m attempting to input image stacks in .dv file format into CellProfiler. The ability of CP to recognize these files as image stacks and extract metadata from the image file headers is inconsistent. I’m able to extract metadata from .dv files from one directory, but if I move the files to another directory, I get an exception in CP core processing error and the files are not recognized as image stacks. I’ve attached a screenshot showing this error output when I attempt to update metadata. You’ll notice that the same file is listed on lines 2 and 3 in the bottom box - it’s simply in different directories. I have uploaded my pipeline too, but have had no luck uploading a sample .dv file - possibly due to file size, each file is ~8 MB. I haven’t been able to troubleshoot this particular problem myself, so any suggestions would be appreciated.
LiveDeadBacteriaAnalysis.cpproj (860 KB)