Evaluation of SARS Staining for COVID-19

Hi,

we’re trying to establish a SARS-antibody staining for COVID-19 in lung tissue. We have difficulties to distinguish the SARS-antibody positive cells (red) from erythrocytes (yellow) and anthracosis (black).

We are grateful for any help.

Kind regards,
Maurice

Original Stain https://www.dropbox.com/s/poc6ams4ppchzxp/3.tiff?dl=0

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There are a few other posts here on color vectors, have you tried this first? You will need pure OD sum for the black, however, as that has effectively lost any other color information (or it is all colors, depending).


More information in the documents here:https://qupath.readthedocs.io/en/latest/docs/tutorials/separating_stains.html#brightfield-images

If you can provide a working example, someone might be able to provide a bit more specific assistance.

Alternatively, you could try training a machine learning classifier based off of the measurements you have, or add more useful features using Add intensity features or other Features.