I’ve been trying to get this pipeline (i.e. classify treatments that would induce lipolysis in adipocytes) of mine to work. So far, CellProfiler has worked fairly well to get to where I need to get to (CellProfiler Analyst). My use for CPA was to
A.) To classify which cells would be differentiated
B.) To compare lipid droplet size (i.e. increase/decrease) when compared to control.
When I try to classify and score cells I always run into errors when I attempt to “Score All”. It never fails to give me the following error:
So, I’m a little confused as to how to precede in using CPA for quantification purposes. At the moment, I’m re-running my CP pipeline where I’ve changed the perimeters of my ExportToDatabase module to produce a “table per object type” thinking that might alleviate the issue. As for now, any help or guidance will be useful (will provide properties file and pipeline as well if needed).