I am trying to use CellProfiler to increase the intensity of my IMC images for processing in ilastik. My preliminary pipeline consists of the following steps:
SmoothMultichannel to remove hot pixels
MeasureImageIntensity with custom percentiles specified (1,99)
RescaleIntensity with the Divide each image by a previously calculated value method selected. The category is Intensity and the Percentile_99 measurement selected.
SaveImages to a 16-bit tiff
The following message appears:
I can fix the error by using the RescaleIntensity module with the Stretch each image to use the full intensity range option selected. However, this removes the desired brightness acheived with the initial option.
Is there a way to save the bright images created with the Percentile_99 (Intensity) option selected that does not trigger this error?
Thanks in advance!