So I’m a relative newbie to CellProfiler, but I have managed to use it successfully before to get object intensity for immunofluorescence images. I tried using the same pipeline, and I keep getting this error while processing the secondary objects. It identifies the cell nuclei perfectly fine! Then I tried building a new pipeline/project for the same measurement and it is coming with the same error. Any ideas??
30-10-14.cpproj (530 KB)