Hi there, I am analyzing 42 microscopic images per group. Each image is divided into 3 sub images representing 3 fluorescent channels.
I am using
First I identify nuclei and use these primary objects as region of interest for an intensity measurement in the other fluorescent channels.
Next I would like to classify the objects according to the intensity of the two fluorescent channels.
I chose “classify objects” with pair of measurements. There I chose my primary objects, category= intensity, measurement = mean intensity, image = fluorescence channel 2 / 3.
For both measurements I defined custom bins.
When I run the analysis, I am seeing the following error: “boolean index did not match indexed array along dimension 0; dimension is 671 but corresponding boolean dimension is 670”
I am using cell profiler 3.0.0 on macOS.