Error when using Classify Objects in CP3

cellprofiler

#1

Hi there, I am analyzing 42 microscopic images per group. Each image is divided into 3 sub images representing 3 fluorescent channels.
I am using

First I identify nuclei and use these primary objects as region of interest for an intensity measurement in the other fluorescent channels.
Next I would like to classify the objects according to the intensity of the two fluorescent channels.
I chose “classify objects” with pair of measurements. There I chose my primary objects, category= intensity, measurement = mean intensity, image = fluorescence channel 2 / 3.
For both measurements I defined custom bins.

When I run the analysis, I am seeing the following error: “boolean index did not match indexed array along dimension 0; dimension is 671 but corresponding boolean dimension is 670”

I am using cell profiler 3.0.0 on macOS.

Best

Ingo


#2

Hi,

Can you share your pipeline and couple of images with me?

Regards
Hamdah


#3

Hi Hamdah, thank you very much for your response. The issue vanished without any action from my site. I can not tell why, but I am not able to reproduce the problem anymore, so I will flag this issue as solved.

Best regards

Ingo