I’m using BoneJ (Slice Geometry) and analyzing a cross sectional image (all grey, white background, jpg). I get this error when I try to run the slice geometry: “No pixels available to calculate. Please check the threshold and ROI.” I’ve searched Help and surfed the ROI posts on this group, but haven’t found a way forward. I have, however, made sure to check the colour of value of the grey and have set the Bone Min and Bone Max accordingly, so that shouldn’t be the problem.
Okay, I’ve noticed that my jpg image is RGB, but I can’t figure out how to redress this. I tried Image > Convert, but it wouldn’t let me do that.
Me again. I solved it. Image > Type > 8-bit did the trick. Please feel free to add any more elegant or insightful explanations.
Nam the Neophyte!
Best to avoid JPG in image processing - it’s lossy. Try PNG instead, or just uncompressed images.