I downloaded ImageJ, then downloaded DiameterJ according to the instructions of extracting the files from the zip folder and pasting them into the Plugins folder for ImageJ. I open Image J. Since I’m only trying to segment one image, I open that image. Make sure it’s in 8-bit. I open the Plugins drop down menu, select Diameter J Segment (I have both 1-017 & 1-018-- both give me the same error message). The basic Information screen opens, I set the cropping coordinates accordingly.
See below for the segmentation algorithms. But after hitting okay, I choose the directory where I want my images stored then another window pops up with the Log and the error message.
For segmentation algorithms, I’ve tried 1. just checking traditional, nothing else. 2. Leaving the boxes that are already checked. (Stat region merged and mixed). 3. none. 4. Just SRM. All possible combos give me the same error message.
I hope this is clear!