I am experiencing an error while attempting to identify primary objects from OrigGreen:
“Error while processing IdentifyPrimaryObjects: index 713 is out of bounds for axis 1 with size 713”.
I am using a slightly modified example “speckles” pipeline, i.e., nuclei size has been changed, but no other modifications have been made with the exception of changing the OrigGreen file identifier to bp1.
I have successfully used this pipeline on a separate image set and am lost as to why I can’t identify the speckles. Please advise.
I have attached sample files.