Hi, I am a new user of CellProfiler. When I download the examples from the web, everything works fine. However, when I try using the same pipelines as templates with my images I get always the same errors: “Could not load file as an image”, or: “Test mode disabled because there are no image sets”. Pipeline and images files are in the same folder. My images are two-color composite .tif pictures from cells immunostained with DAPI (blue) and a specific Alexa fluor Ab (green).
I tried using several pipelines from your web (ColorToGray, Example%positive…) with my images and always get the same message. I´ve been reading some other posts and I guess there is a problem with NamesAndTypes but I do not know how to solve it. In the manual only says to load ones images.
Could someone give me a step by step walkthrough on this? Thanks a lot!