Attached, you will find an example grayscale image of a pretty ineffective membrane stain.
Unfortunately, we cannot redo this stain due to lack of tissue. I need to enhance the membrane while removing the noisy color within the interior of the cell body. I have tried a variety of methods, but was hopeful you could provide a superior method as these attempts have been largely unsuccessful. I need to measure the area of the cell cell body and to do so, I need a pronounced membrane so that the cell bodies are separated appropriately.
Any help would be much appreciated!